Isihluzi seFluorescence yinxalenye ephambili esetyenziswa kwizixhobo zenzululwazi yebhayoloji kunye nenzululwazi yobomi.Umsebenzi wayo ophambili kukwahlula kwaye ukhethe uphawu lwe-wavelength spectrum ukusuka kwisibane esivuselelayo kunye nokukhutshwa kwe-fluorescence ye-substance kwi-biomedical fluorescence yokuhlola kunye nenkqubo yokuhlalutya.Izihluzi ze-Fluorescence zibonakaliswa ngobunzulu obusikiweyo obunzulu kunye ne-autofluorescence ephantsi.Ngesiqhelo, izihluzi ezininzi zinokudityaniswa kunye ukwenza isihluzo se-fluorescence, esinexabiso eliphezulu lokusebenza.
Isihluzi seFluorescence sisetyenziswa kwixesha lokwenyani le-fluorescence quantitative PCR isixhobo.esetyenziswa kakhulu kwibhayoloji yemolekyuli kunye nokufumanisa ukhuseleko lokutya kunye nokubeka iliso kubhubhane lwempilo yoluntu kunye namanye amashishini.
Isihluzi seFluorescence sisetyenziselwa ukukhangela iindidi zedayi kunye neprobe, umzekelo:
FAM/SYBR Green/Green/ HEX/TET/Cy3/JOE/ROX/Cy3.5/Texas Red,Cy5/LC Red640,Cy5.5 njl.
Inkqubo | (IAD iNgqungquthela) | ||
Ubude bamaza | Ex(nm) | Em(nm) | Umnqamlezo |
| 470-30 | 525-20 | >6 |
| 523-20 | 564-20 | >6 |
| 543-20 | 584-20 | >6 |
| 571-20 | 612-20 | >6 |
| 628-35 | 692-45 | >6 |
Ukuthintela | OD>6@200~900nm okanye @200~1200nm | ||
Ithambeka(nm) | 50 %~ OD5 <10nm | ||
Umnqamlezo | OD>6 | ||
Ubungakanani(mm) | Φ4mm, Φ12mm,Φ12.7mm,Φ25.4mm njl. |